CAST scientist Rick van den Hurk wrote a review on recent developments in 2D-LC and the use of 2D-LC in industry. He did this under the supervision of Bob Pirok and in collaboration with Matthias Pursh (Dow) and Dwight Stoll (Gustavus Adolphus College).
Two-dimensional liquid chromatography (2D-LC) greatly advances the separation powered of analytical separation sciences through a better peak capacity as well as offering more-tailored selectivity combinations.
However, the field of 2D-LC is, in particular in contrast to 2D-GC, still very immature and under significant development. In 2019, Bob Pirok, Dwight Stoll and Peter Schoenmakers published a review in which they examined the latest trends from 2015 until 2019 [1].
In this recent installment [2], Rick van den Hurk reviewed the recent innovations between 2019 and 2023. In addition the review also devotes significant focus to the implementation of the technique in industry. The review was co-written by Bob Pirok, Dwight Stoll (Gustavus Adolphus College) and Matthias Pursch (Dow).
The authors examined over 200 articles and also compared these with the articles published prior to 2019. In their review, the authors concluded that mobile-phase mismatch continues to be an important focus area for the field, and several modulation strategies and new variants were discussed.
Van den Hurk and co-workers also noticed that a third of the publications had at least one author affiliated with industry. Application fields that particularly demonstrated involvement were the polymer characterization, metabolomics, and pharmaceutical and biopharmaceutical analysis. Furthermore, industrial applications favored the use of heart-cut 2D-LC and largely employed on-line hyphenation. The authors did note that the database was likely to be missing out on a number of industrial works that are not published for confidentiality reasons.
Other important developments were the increased popularity of computer-aided strategies, alternative gradient-elution methods to facilitate modulation, as well as multi-stage, multi-dimensional separations, the latter of which were applied to the characterization of protein therapeutics.
Two-dimensional liquid chromatography is of paramount importance to the PARADISE project of CAST scientist Bob Pirok in which multi-dimensional separation technology is used to achieve separation of highly complex samples. In addition, the project aims to characterize the correlation of different sample properties within a single analytical solution. The outcomes of this recent review are thus of value to the ongoing progress in the PARADISE project. Rick van den Hurk is a PhD candidate in the PARADISE project, which stands for Propelling Analysts by Removing Analytical-, Data-, Instrument- and Sample-related Encumbrances and receives funding from the Dutch Research Council (NWO), as well as a number of public and private organisations. Read more about the PARADISE project here.
In addition, the review was part of Pirok’s UPSTAIRS project, which aims to improve the accessibility of advanced separation technology by developing computational methods to leverage chromatographic theory in an unsupervised workflow. This project also receives funding from NWO.
The work was published in TrAC Trends in Analytical Chemistry as open access, and can thus be accessed for free here.
References
- Recent Developments in Two-Dimensional Liquid Chromatography: Fundamental Improvements for Practical Applications, B.W.J. Pirok, D.R. Stoll and P.J. Schoenmakers, Anal. Chem., 2019, 91(1), 240-263, DOI: 10.1021/acs.analchem.8b04841
- Recent trends in two-dimensional liquid chromatography, R.S. van den Hurk, M. Pursch, D.R. Stoll, B.W.J. Pirok, TrAC Trends in Analytical Chemistry, 2023, 166, 117166, DOI: 1016/j.trac.2023.117166
