Welcome to the website of the Chemometrics & Advanced Separations Team (CAST) of Bob Pirok, Andrea Gargano, and Tijmen Bos, with researchers from the University of Amsterdam and VU University Amsterdam. The team comprises enthusiastic researchers from both universities who devote a bit of their time to developing guides and tools for use by the community. Here, you will be able to find useful, information, software and tools for use in mass spectrometry and (multi-dimensional) chromatography.
In this study, CAST scientists Annika van der Zon et al., in collaboration with Bram Weijers and Danielle Vlugts (Amsterdam UMC), introduce a rapid, microscale aqueous size-exclusion chromatography–mass spectrometry (SEC-MS) method to measure chelator to antibody ratio in immunoconjugates.
The study addresses size-resolved surface charge density (SCD) analysis of polymer nanoparticles (PNPs), revealing the significant variability of SCD that traditional methods overlook. By employing advanced capillary zone electrophoresis techniques, researchers can gain detailed insights into nanoparticle performance, benefiting applications in coatings, biomedical, and industrial contexts through collaborative innovations.
As multidimensional chromatography continues to expand its reach across complex analytical challenges, the precision of data interpretation increasingly depends on what happens after separation. In
As analytical chemistry continues to push the limits of resolution and complexity, even subtle physical effects can determine the quality of results. A recent study
Ervin sz. Kováts Award for Young Scientists Awarded to Dr. Tijmen S. Bos At the 14th Balaton Symposium on High-Performance Separation Methods, held this
CAST scientist Luca Tutiš together with Govert Somsen and Andrea Gargano as well as AstraZeneca, Novartis and GSK have just published a manuscript describing a novel LC-MS method for impurity profiling of therapeutic phosphorothioated oligonucleotides.
You can download some of our developed software and tools here. The overview is not exhaustive. We have only recently begun with making our work accessible. Currently a manual only exists for the FILTER tool. More apps, manuals and information repositories will be added through September and October.
Visit the special page on the public-privately funded PARADISE project. The project aims to resolve pressing matters in industry and society using 2D-LC. The project is funded by a consortium of Covestro, DSM, Genentech, NFI, Shell and the Dutch Research Council (NWO).
We maintain a searchable database containing all 2D-LC methods published since the 1978 paper by Erni and Frei. The database, which contains over 550 methods, is part of a collaboration between the University of Amsterdam (Pirok) and Gustavus Adolphus College (Stoll).
Researchers Rick van den Hurk, Dr. Tijmen Bos, and Dr. Bob Pirok have, together with scientists Dr. Ynze Mengerink (Brightlands Chemelot Campus) and Prof. Dr.
CAST scientists Annika van der Zon and Ziran Zhai have just published two manuscriptsshowcasing significant advances in the low flow analysis of intact antibodies and
Scientists from the CAST team and related projects within the Center of Analytical Science Amsterdam actively participated with lectures, posters and social contributions to the HPLC2025 conference in Brugge, Belgium.